Journal: bioRxiv

Article Title: Monocytes Strongly Induce (MYO)Fibroblast Contraction in a New 3D Skin Model to Understand the Inflammation-Fibrosis Axis in Systemic Sclerosis

doi: 10.64898/2026.02.12.705496

Figure Lengend Snippet: ( A ) Representative immunohistochemistry staining showing clear CD68 expression in the monocyte-containing hydrogels (right). Black arrows indicate a “surrounding layer” formed by the CD68 + cells covering the fibroblasts. ( B ) Flow cytometry analyses showing the percentage of total macrophages (CD45 + CD68 + ) isolated from the hydrogels after enzymatic digestion and subsequently CD45 + magnetic selection. Cells (CD45 + CD68 + ) were classified into M1-like (CD163 − ) or M2-like (CD163 + ) macrophages. Comparisons were performed using Student’s t-test (*p<0.05). Each shape represents the average of 3 technical replicates of 1 PBMC donor, n = 3.

Article Snippet: Sections were then incubated with primary rabbit –anti-human alpha-smooth muscle actin (α-SMA) (1:200) (ab5694, Abcam, UK), – fibroblast activation protein (FAP) (1:100) (ab207178, Abcam), – Phospho-SMAD2 (1:100) (#3108L, CellSignaling, USA), – Phospho-Stat3 (1:50) (#9145, CellSignaling), or mouse anti-human – CD68 (#MCA1815, BioRad, USA) (1:200) antibodies, for 1 hour at room temperature.

Techniques: Immunohistochemistry, Staining, Expressing, Flow Cytometry, Isolation, Selection

Journal: Clinics

Article Title: M2-Polarized tumor-associated macrophages are associated with poor prognoses resulting from accelerated lymphangiogenesis in lung adenocarcinoma

doi: 10.1590/S1807-59322011001100006

Figure Lengend Snippet: M2- and M1-polarized TAMs in lung adenocarcinoma. (A) The coexpression of CD68 and MMR in TAMs in human lung adenocarcinoma sections. (B) The coexpression of CD68 and iNOS in TAMs in human lung adenocarcinoma sections.

Article Snippet: Next, slides were immunostained using a monoclonal mouse anti-human CD68 antibody (1∶100; Zhongshan Company, Beijing, China).

Techniques:

Journal: BMC Cancer

Article Title: Local iron homeostasis in the breast ductal carcinoma microenvironment

doi: 10.1186/s12885-016-2228-y

Figure Lengend Snippet: FPN1-expressing leukocytes in carcinomas are predominantly M2-like. Sections of normal breast tissue, DCIS (ductal carcinoma in situ) and IDC (invasive ductal carcinoma) to reveal the presence of cells of the macrophage lineage (CD68), and its classical polarization phenotypes, M1-like (CD80) and M2-like (CD163), in FPN1-expressing leukocyte infiltrate. For details see Materials and Methods (Original magnification × 100- upper CD68 images, ×400- squared image series)

Article Snippet: Immunohistochemical staining was performed in 2 μm-thick TMA sections with the following antibodies: rabbit polyclonal anti-human hepcidin-25 antibody (dilution 1:500, Abcam, Cambridge, UK [ ]), rabbit polyclonal anti-human ferroportin 1 antibody (FPN—1:500, Novus Biologicals Europe, Cambridge, UK [ ]), rabbit polyclonal anti-human ferritin antibody (FT—1:1000, Sigma-Aldrich, MO, USA [ ]), mouse monoclonal anti-human CD71 (TFR1 [clone 10 F11]- 1:80, Novocastra, Newcastle, UK [ ]), mouse monoclonal anti-human CD68 (clone Kp-1, 1:2000, A. Menarini Diagnostics, CA, USA), mouse monoclonal anti-human CD163 (clone MRQ-26, 1:100, Cell Marque, CA, USA), mouse monoclonal anti-human CD80 (37711, 1:100, R&D Systems, MN, USA), rabbit polyclonal anti-human CD4 (clone H-370, 1:250, Santa Cruz Biotechnology, TX, USA) and mouse monoclonal anti-human CD8 (clone C8/144B, 1:100, Cell Marque, CA, USA).

Techniques: Expressing, In Situ

Journal: BMC Cancer

Article Title: Local iron homeostasis in the breast ductal carcinoma microenvironment

doi: 10.1186/s12885-016-2228-y

Figure Lengend Snippet: Representative images of the FPN1 analysis by Imaging Flow Cytometry in breast cancer core biopsies. Epithelial cells (EC) are stained by an anti-cytokeratin (CK) FITC. T lymphocytes (T Ly) were identifiable by CD3 PerCP-Cy5.5, B lymphocytes (B Ly) by CD20 PE-Cy7 and macrophages (M0) by CD68 PE-Cy7 (Original magnification × 400)

Article Snippet: Immunohistochemical staining was performed in 2 μm-thick TMA sections with the following antibodies: rabbit polyclonal anti-human hepcidin-25 antibody (dilution 1:500, Abcam, Cambridge, UK [ ]), rabbit polyclonal anti-human ferroportin 1 antibody (FPN—1:500, Novus Biologicals Europe, Cambridge, UK [ ]), rabbit polyclonal anti-human ferritin antibody (FT—1:1000, Sigma-Aldrich, MO, USA [ ]), mouse monoclonal anti-human CD71 (TFR1 [clone 10 F11]- 1:80, Novocastra, Newcastle, UK [ ]), mouse monoclonal anti-human CD68 (clone Kp-1, 1:2000, A. Menarini Diagnostics, CA, USA), mouse monoclonal anti-human CD163 (clone MRQ-26, 1:100, Cell Marque, CA, USA), mouse monoclonal anti-human CD80 (37711, 1:100, R&D Systems, MN, USA), rabbit polyclonal anti-human CD4 (clone H-370, 1:250, Santa Cruz Biotechnology, TX, USA) and mouse monoclonal anti-human CD8 (clone C8/144B, 1:100, Cell Marque, CA, USA).

Techniques: Imaging, Flow Cytometry, Staining